Publications

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Korolnek T, Hamza I. Macrophages and iron trafficking at the birth and death of red cells. Blood. 2015 May 7; 125(19): 2893-7. Review. PubMed ID: 25778532.

For this publication, I performed an in-depth review of historical and recent literature on the subject of iron homeostasis in red blood cells and relevant populations of macrophages. My main goal for this publication was the dissemination of recent discoveries in iron and heme-iron metabolism to an audience of clinical and experimental hematologists that are the primary audience of Blood (which is published by ASH – the American Society of Hematology).

 

Korolnek T, Hamza I. Like iron in the blood of the people: the requirement for heme trafficking in iron metabolism. Frontiers in Pharmacology. 2014 Jun 4; 5:126. Review. PubMed ID: 24926267.

This review was requested from my PhD advisor by editors at Frontiers in Pharmacology for their research topic “The Importance of Iron in Pathophysiologic Conditions.” I performed an in-depth synthesis of the literature to summarize the evidence that heme-iron requires a network of (as-yet-undiscovered) transporters and chaperones to be mobilized within cells and across tissues. This focus was unique, as study of heme-iron transport has historically been neglected in favour of studying molecular iron transport.

 

Korolnek T, Zhang J, Beardsley S, Scheffer GL, Hamza I. Control of metazoan heme homeostasis by a conserved multidrug resistance protein. Cell Metabolism. 2014 Jun 3; 19(6): 1008-19. PubMed ID: 24836561.

This publication was the result of my doctoral dissertation work performed under the supervision of Dr. Iqbal Hamza. This study delineated the role of an ABC transporter in heme-iron metabolism, beginning with an analysis of the role it plays in C. elegans and expanding its importance to zebrafish and mammalian model systems. As of July 2016, this research article has been cited by 10 Pubmed Central articles.

 

Banaudha K, Kaliszewski M, Korolnek T, Florea L, Yeung ML, Jeang KT, Kumar A. MicroRNA silencing of tumor suppressor DLC-1 promotes efficient hepatitis C virus replication in primary human hepatocytes. Hepatology. 2011 Jan; 53(1): 53-61. PubMed ID: 20967756.

As a master’s student in Dr. Ajit Kumar’s laboratory at George Washington University, I helped uncover a microRNA pathway that is misregulated during hepatitis C infection. Dr. Krishna Banaudha developed a method for growing primary hepatocytes in culture, and had shown that these cells could support Hepatitis C virus (HCV) infection. A previous graduate student had performed microarray analysis of microRNA expression that is altered in these HCV-infected cells and I performed the target validation experiments.

 

Banaudha K, Orenstein JM, Korolnek T, St Laurent GC 3rd, Wakita T, Kumar A. Primary hepatocyte culture supports hepatitis C virus replication: a model for infection-associated hepatocarcinogenesis. Hepatology. 2010 Jun; 51(6): 1922-32. PubMed PMID: 20512986.

This publication was the result of research I performed as a new master’s student in Dr. Ajit Kumar’s laboratory at George Washington University. Dr. Krishna Banaudha, a staff scientist in the lab developed a method for growing primary hepatocytes in culture, and showed that these cells could support HCV infection. For this publication, I performed the Western blots for HCV proteins seen in the supplementary figures.

 

Tubes

Primers for sequencing a 7000 base pair open reading frame